Discovery Could Lead to More Effective Prevention, Treatments
New research by a team including Distinguished University Professor Rita Colwell found that microbial changes in the intestine of babies at risk of celiac disease can provide an early warning before symptoms develop, allowing early intervention.
Substantial microbial changes found in the intestines of infants at risk of celiac disease indicate which will develop the autoimmune disorder as early as 18 months before its onset, according to new study including a University of Maryland researcher.
Using advanced genomic sequencing techniques, the researchers including Distinguished University Professor Rita Colwell uncovered distinct alterations in several species of microorganisms and molecular components of cells and tissues in children who developed celiac disease that were absent in at-risk children who did not.
Results of the study, published July 12 in Proceedings of the National Academy of Sciences, could lead to more effective celiac disease treatments and prevention.
“These results indicate that the microbiome can be a powerful indicator for celiac disease,” said Colwell, whose appointment is in the University of Maryland Institute for Advanced Computer Studies. “It provides an early warning before symptoms develop, allowing early intervention. With analysis of the microbiome via stool samples, infants can be monitored, and it may be possible for alteration of the diet to be sufficient to treat or prevent the disease.”
Celiac disease is a serious autoimmune condition in which the consumption of gluten leads to damage in the small intestine. It is estimated to affect one in 100 people worldwide.
Colwell and the research team tracked the gut microbiota of 500 at-risk children from birth through age 10 as part of the MassGeneral Hospital for Children (MGHfC) Celiac Disease, Genomic, Microbiome and Metabolomic study (CDGEMM). They began collecting extensive blood and fecal samples along with environmental data on participants in 2014. Using metagenomic analysis, the researchers linked microbial composition with function and highlighted changes associated with either increased inflammatory processes or reduced inflammation. An important part of the body’s immune response, inflammation is a significant cause of celiac disease symptoms.
For the new paper, the team compared the gut microbiome of 10 infants from the CDGEMM study who went on to develop celiac disease with the gut microbiome of 10 infants from the study who did not. All 20 were genetically predisposed to develop celiac disease.
“We found significant changes in the intestinal microbes, pathways and metabolites 18 months before disease onset, which was confirmed with positive lab tests,” said Maureen Leonard, M.D., lead author of the study and clinical director of the Center for Celiac Research and Treatment at MGHfC. “This was much earlier than we expected.”
The changes researchers found included increases in pro-inflammatory microorganisms and decreases in protective and anti-inflammatory microorganisms at various time points before onset of the disease.
Colwell said the study demonstrates the power of next-generation sequencing coupled with bioinformatics to detect these important changes. It was partially supported by the National Institutes of Health’s National Institute of Diabetes and Digestive and Kidney Diseases, the Nutrition Obesity Research Center at Harvard, the Thrasher Research Fund, faculty startup funding by Mucosal Immunology and Biology Research Center at Massachusetts General Hospital, and Joyce and Hugh McCormick.
According to the paper’s senior author, Alessio Fasano, M.D., director of the Center for Celiac Research and Treatment, the approach the team used will help researchers develop similar studies for the diagnosis and treatment of a variety of conditions in which the microbiome could play a pathogenic role.
If confirmed by larger datasets, these findings may represent specific therapeutic targets for disease interception and possible prevention of celiac disease onset through microbiome manipulation during the preclinical phase.
This story was adapted from text provided by MassGeneral Hospital for Children.
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